Quantification and characterization of airborne microplastics in the coastal area of Terengganu, Malaysia.

Microplastics (MPs) pose a threat to ecosystems due to their capacity to bind with toxic chemicals. While the occurrence of MPs in aquatic environmental matrices like water, sediments, and biota is well studied, their presence in the atmosphere remains less understood. This study aimed to determine the presence of airborne MPs and their characteristics through ground-based sampling in the coastal city of Kuala Nerus, Terengganu, Malaysia. Airborne MP samples were collected using passive sampling technique in December 2019. MPs were manually counted and identified using a stereomicroscope based on their colour and shape. The average deposition rate of airborne MPs during the sampling period was 5476 ± 3796 particles/m2/day, ranging from 576 to 15,562 particles/m2/day. Various colours such as transparent (38%), blue (25%), black (20%), red (13%), and others (4%) were observed. The predominant shape of airborne MPs was fibres (> 99%). The morphology structure of MPs observed using a scanning electron microscope (SEM) showed a cracked surface on MPs, suggesting weathering and irregular fragmentation. Further elemental analysis using energy dispersive X-ray spectroscopy (EDS) showed the presence of heavy metals such as aluminium (Al) and cadmium (Cd) on the surface of MPs, attributed to the adsorption capacities of MPs. Polymer types of airborne MPs were analysed using attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR), which revealed particles composed of polyester (PES), polyethylene (PE), and polypropylene (PP). The preliminary findings could provide additional information for further investigations of MPs, especially in the atmosphere, to better understand their sources and potential human exposure.

Vertical distribution of microplastic along the main gate of Indonesian Throughflow pathways.

Even though Pacific - Indian Ocean exchange [Indonesian Throughflow (ITF)] has been measured for the last three decades, the measurements of microplastic in the region is very limited. This study was the initial investigation of the vertical distribution of microplastic in the deep-sea areas across the ITF Pathway. Niskin water samples were utilized to obtain the samples from a water column in a range of 5 to 2450 m. A total of 924 microplastic particles with an average abundance of 1.062 ± 0.646. n/L were found in the water column. Our findings indicate that water temperature and water density are the most significant factors correlated to the microplastic concentration. This study will be the first report discussing the distribution of microplastics in the deep-sea water column that could be highly significant in determining the fate and transport of microplastic within Indonesian waters that exits into the Indian Ocean.

First evidence of microplastic pollution in the surface water of Malaysian Marine Park islands, South China Sea during COVID-19.

Malaysia is bounded by the South China Sea with many islands that support species megadiversity and coral reef ecosystems. This study investigates the distribution of microplastics (MPs) in the surface water around the four marine park islands (Perhentian, Redang, Kapas, and Tenggol) during COVID-19. The global pandemic has reset human activities, impacting the environment while possibly reducing anthropogenic contributions of microplastic pollution near the South China Sea islands. It was found that Pulau Perhentian recorded the most abundance of MPs (588.33 ± 111.77 items/L), followed by Pulau Redang (314.67 ± 58.08 items/L), Pulau Kapas (359.8 ± 87.70 items/L) and Pulau Tenggol (294.33 ± 101.64 items/L). Kruskal-Wallis analysis indicates a significant difference in total MPs abundance between islands. There are moderate correlations between salinity, pH, temperature and MPs variability. Among these parameters, only temperature is significant (p < 0.05) as proven by the principal component analysis and multiple linear regression analysis. Nearly 99 % of MPs are fiber type, with the majority of them being black and transparent. Micro-FTIR spectroscopy revealed polyethylene, polypropylene, polyvinyl methyl ether, polyamide, phenoxy-resins and polyurethane-acrylic are associated with MPs. The findings provide a new baseline reference for the MPs distribution on Malaysian islands, which contributes to a potential future direction regarding marine sustainability.

A multidimensional approach for microplastics monitoring in two major tropical river basins, Malaysia.

Microplastics (MPs) with the size of 1 µm-5 mm are pollutants of great concern ubiquitously found in the environment. Existing efforts have found that most of the MPs present in the seas mainly originated from land via riverine inputs. Asian rivers are known to be among the top in microplastic emissions. However, field data are scarce, especially in Malaysia. This study presents the distribution and characteristics of MPs in the surface water of two major river basins of Malaysia, namely Langat River (West Coast/Straits of Malacca) and Kelantan River (East Coast/South China Sea). Water samples were collected at 21-22 locations in Kelantan and Langat rivers, covering the river, estuary and sea. MPs were physically classified based on sizes, shapes, colours and surface morphology (SEM-EDS). The average of 179.6 items/L and 1464.8 items/L of MPs had been quantified from Kelantan and Langat rivers, respectively. Fibre (91.90%) was highly recorded at Kelantan, compared to Langat whereby both fibre (59.21%) and fragment (38.87%) were prevalence. Anthropogenic activities and urbanised areas contribute to high microplastic abundance, especially in the Langat River. Micro-FTIR analysis identified 14 polymers in Kelantan River, whereas 20 polymers were found in Langat River. Polypropylene, polyethylene, polyethylene terephthalate, nylon, phenoxy resins, poly(methyl acrylate), poly(methyl methacrylate), polystyrene, polytetrafluoroethylene, polyurethane and rayon were discovered in both rivers, although only polyethylene was significant (>1 ppm) when further analysed using pyrolysis-GC/MS. Correlation analysis and multiple linear regression were used to explain the relationship between water quality and MP abundance, suggesting only turbidity was positively significant to the microplastic occurrence. This comprehensive study is first to suggest a full-scale monitoring protocol for MPs in Malaysian riverine system and is significant in understanding MPs abundance in correlation to in-situ environmental factors. Consequently, this will allow the right authorities to develop mitigation strategies to address riverine plastic pollution in major river basins in Malaysia and the South East Asia.

Utilizing Pyrolysis-Gas Chromatography/Mass Spectrometry for Monitoring and Analytical Characterization of Microplastics in Polychaete Worms.

Microplastics (the term for plastics at sizes of <5 mm) might be introduced into the environment from domestic or agricultural activities or from the breakdown of plastic pieces, particles, and debris that are bigger in size. Their presence in the aquatic environment has caused accumulation problems, as microplastics do not easily break down and can be digested by some aquatic organisms. This study was conducted to screen and monitor the level of microplastic pollution in polychaete worms using pyrolysis−gas chromatography/mass spectrometry (Py-GC/MS). The study was conducted in Setiu Wetlands, Malaysia from November 2015 to January 2017 at five-month intervals and covered all monsoon changes. Results from physical and visual analyses indicated that a total number of 371.4 ± 20.2 items/g microplastics were retrieved from polychaete for all seasons, in which, the majority comprised transparent microplastics (49.87%), followed by brown with 138.3 ± 13.6 items/g (37.24%), 21.7 ± 1.9 items/g for blue (5.84%), and 12.9 ± 1.1 items/g for black (3.47%), while the remaining were green and grey-red colors. Statistical analysis using Kruskal−Wallis showed insignificant differences (p > 0.05) between the sampling station and period for the presence of a microplastics amount. Most of the microplastics were found in fiber form (81.5%), whereas the remaining comprised fragment (18.31%) and film (0.19%) forms. Further analysis with Py-GC/MS under a selective ion monitoring mode indicated that pyrolytic products and fragment ions for a variety of polymers, such as polyvinyl chloride, polypropylene, polyethylene, polyethylene terephthalate, polyamide, and polymethylmethacrylate, were detected. This study provides an insightful application of Py-GC/MS techniques for microplastics monitoring, especially when dealing with analytical amounts of samples.

The degradation and toxicity of commercially traded vegetable oils following spills in aquatic environment.

The production of commodity and specialty vegetable oils is increasing every year to fulfill the ever-increasing demand where the trading of oils occurs primarily via sea shipping. Spills of vegetable oil into the aquatic environment may result in detrimental effects on aquatic ecosystems. Environmental degradation of vegetable oil spills occurs mainly via microbial activity, chemical oxidation, wave and wind actions. However, the polymerization of oils can hinder their ability to naturally degrade. Thus, human intervention in the form of both short- and long-term remediation, is desirable to reduce the effects of vegetable oil spills on aquatic ecosystems. Studies have been conducted to determine how the type and concentration of the vegetable oil contamination influence its toxicity on various organisms. Some studies show that the effect of vegetable oil spills is found to be relatively short-lived and to a certain extent increase the survivability of certain organisms. However, the integrated effect of vegetable oil spills on aquatic organisms and their environment is still being researched. This review summarizes the existing knowledge on the reported occurrences of vegetable oil spills, their degradation, and their toxicity towards the surrounding aquatic environment which would be helpful in the knowledge transfer of remediation of vegetable oils.

Ingestion of microplastics by the estuarine polychaete, Namalycastis sp. in the Setiu Wetlands, Malaysia.

In this study, the ingestion of microplastics by the deposit-feeding polychaete Namalycastis sp. in the estuarine area of the Setiu Wetlands, Malaysia was confirmed. Samples were collected from six stations, covering the wetland from the south to the north, bimonthly between November 2016 and November 2017. Microplastics were extracted from polychaete samples following digestion in an alkaline solution (10 M NaOH). They were identified by physical characteristics (i.e., shape and color under dissecting microscope and scanning electron microscope), and chemical analysis using a LUMOS Fourier Transform Infrared Microscope (µ-FTIR). A total of 3277 pieces were identified, which were dominated by filaments (99.79%) and with the majority transparent in color (84.71%). Most of the microplastics identified were polypropylene (PP) followed by polyamide (PA) based on their main peak in the of µ-FTIR spectrum. Principal component analysis demonstrated the dominance of microplastics at stations 3 and 4 of the sampling area, probably because of the influx from the open sea and from aquaculture. The findings of this research provide baseline information on microplastics ingested by benthic organisms and their fate in the estuarine food web.

Microplastics in seawater and zooplankton: A case study from Terengganu estuary and offshore waters, Malaysia.

Widespread accumulation and distribution of microplastics at the sea surface raise concerns as the habitat is a feeding ground for zooplankton. As primary consumers, these organisms are closely connected to microplastic input in the marine food chain. Little comparative information currently exists about this problem in estuary and offshore systems. This study investigates microplastic distribution in the surface water and the potential ingestion of microplastics in selected taxonomic groups of zooplankton from the Terengganu Estuary to offshore waters, Malaysia. In the surface water, three types of microplastics were found (fibres, fragments and pellets). Fibres made up the highest percentage, comprising 80.8% and 73.8% of microplastics in offshore waters and estuaries, respectively. The highest total density of microplastics was found in the Terengganu Estuary (545.8 particles m-3). Microplastics sampled from the offshore waters were identified as polyamide, polyethylene, and polypropylene, which possibly originated from secondary microplastic sources. Two types of microplastics were detected in zooplankton: fibres and fragments. Fibres were the most commonly ingested microplastic type in zooplankton collected from offshore waters (94%) and estuaries (77.7%). The average sizes of ingested fibres and fragments were 361.7 ± 226.8 µm and 96.8 ± 28.1 µm, respectively, with a wider range of sizes ingested observed in offshore waters than in estuaries. The concentration of microplastics in seven zooplankton groups varied from 0.01 ± 0.002 particles ind.-1 (Harpacticoida) to 0.2 ± 0.14 particles ind.-1 (Aphragmophora). Notwithstanding the conformity of our results (increased anthropogenic activities led to greater plastic pollution within the estuary), no significant correlation was observed between the levels of microplastic ingestion and microplastic concentration in the surface water within both areas. Our results provide an important baseline reference on microplastic pollution from estuary to offshore waters, as well as proving that zooplankton act as a repository for microplastic in the marine ecosystem.

Spatiotemporal microplastic occurrence study of Setiu Wetland, South China Sea.

This study reports the distribution of microplastics (MPs) in surface water and estuarine sediments in South and North Setiu Wetland in the South China Sea. Sampling was conducted bimonthly for one year from November 2016 to November 2017, including the northeast and southwest monsoons. Water surface and sediment samples were collected from six different sampling stations (STs). Samples were sorted based on physical analysis (optical observation) and selected particles were further analyzed by chemical characterizations. The findings of this study indicate that a total of 0.36 items/L and 5.97 items/g particles of MPs were found from characterizations surface water and dry sediment, respectively. Among the selected stations included in this research, ST3 (1.375 ± 0.347 items/L) and ST2 (14.250 ± 4.343 items/g) were individually identified as high potential MP sinking areas, exacerbated during the northeast and southwest monsoons. Transparent, film, and filament MP types were consistently found across all stations. Microplastic filaments revealed a functional group of polypropylenes based on the main peak spectrum at 2893-2955 cm-1 (CH alkyl stretching), 1458 cm-1 (CH2 bending), and 1381 cm-1 (CH3 bending). Microplastic materials were thermally decomposed by pyrolysis-gas chromatography-mass spectrometry (Pyr-GC/MS) and identified as cyclohexane and cyclohexene derivatives, as well as precursors of polymer blends. The distribution of MPs in both matrices varied according to different seasons. These findings provide useful baseline information on the distribution of MPs from the estuarine area in Malaysia and South China Sea waters.

Microplastic ingestion by zooplankton in Terengganu coastal waters, southern South China Sea.

This study investigates the presence of microplastics in surface seawater and zooplankton at five different locations off the Terengganu coast in Malaysia, southern South China Sea. A total of 983 microplastic particles, with an average abundance of 3.3 particles L-1 were found in surface seawater. An average of one plastic particle was detected in 130 individuals from 6 groups of zooplankton. These groups include fish larvae, cyclopoid, shrimps, polychaete, calanoid and chaetognath where they ingested 0.14, 0.13, 0.01, 0.007, 0.005 and 0.003 particle per individual, respectively. Microplastics in the form of fragments are the most common type of ingested microplastics that ranged between 0.02 mm (cyclopoid) - 1.68 mm (shrimp and zoea). Contrastingly, fibers, which are identified as polyamide are the main type of microplastics that dominate in seawater.

The development of flow-through bio-catalyst microreactors from silica micro structured fibers for lipid transformations.

This study demonstrates the utility of a flow-through enzyme immobilized silica microreactor for lipid transformations. A silica micro structured fiber (MSF) consisting of 168 channels of internal diameter 4-5 µm provided a large surface area for the covalent immobilization of Candida antartica lipase. The specific activity of the immobilized lipase was determined by hydrolysis of p-nitrophenyl butyrate and calculated to be 0.81 U/mg. The catalytic performance of the lipase microreactor was demonstrated by the efficient ethanolysis of canola oil. The parameters affecting the performance of the MSF microreactor, including temperature and reaction flow rate, were investigated. Characterization of the lipid products exiting the microreactor was performed by non-aqueous reversed-phased liquid chromatography (NARP-LC) with evaporative light scattering detector (ELSD) and by comprehensive two-dimensional gas chromatography (GC x GC). Under optimized conditions of 1 µL/min flow rate of 5 mg/mL trioleoylglycerol (TO) in ethanol and 50 °C reaction temperature, 2-monooleoylglycerol was the main product at > 90% reaction yield. The regioselectivity of the Candida antartica lipase immobilized MSF microreactor in the presence of ethanol was found to be comparable to that obtained under conventional conditions. The ability of these reusable flow-through microreactors to regioselectively form monoacylglycerides in high yield from triacylglycerides demonstrate their potential use in small-scale lipid transformations or analytical lipids profiling.